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Master thesis defense

Transcript: Master thesis defense How to Our company Our company The team The team By the numbers By the numbers Global sales Global sales Interaction Interaction History History Today Expanded First major product Established Testimonials Testimonials Products & services Products & services

Defense Presentation - Master Thesis

Transcript: Introduction Developments in Labor Governance Approaches to the Study of Compliance with International Labor Standards Literature Review state hybrid Theory and Hypotheses Institute for Labor Studies Fostering Voluntary Compliance: An Assessment of the Labor Laws Compliance System 2015 Theory: Performance Assessment of Labor Governance Regimes Historical Comparison Research problem and objectives Relevance and importance of the study Summary and Conclusions "Why do firms comply (or do not comply) with international labor standards?" Developments in Labor Governance Findings and Analysis Approaches to the Study of Compliance with International Labor Standards Normative Framework on Workers' Rights and Labor Standards Methodology SINGLE CASE STUDY The operation of the normative framework tightens the network of rules and reduces labor laws circumvention... However, it has not yet fully pushed enforcement of ILS to a regime that goes beyond self and voluntary regulatory strategies as typified in the Philippines... The Changing Behavior of Firms Findings and Analysis self Literature Review Limitations Anke Hassel and Nicole Helmerich Institutional Change in Transnational Labor Governance: Implementing Social Standards in Public Procurement and Export Credit Guarantees 2016 Performance Assessment of Labor Governance Regimes Research hypotheses Rationale (Relevance and Importance of the Study) How the behavior of firms changed in the points of transition to a new labor governance regime despite the existence of international labor standards... even with sophistication of compliance systems... the horrors of the Kentex tragedy still continue to haunt workers under precarious working conditions... Hypotheses Research Design Hypothesis 2 The Philippine government transitioned to a voluntary labor governance regime because of the increasing pressure from transnational standard-setting institutions. This served as a window of opportunity for the ruling administration at the time of transition to re-establish its credibility and relevance in labor governance. Further, contrary to theory (Hurd 2003, Hassel 2008), firms comply more with the traditional regime of labor regulation than the private regime. Further Research Further research should look at the use of other methods Observational methods can be used to establish a causal link on the effect or impact of labor governance regime (mandatory, voluntary or hybrid) to the behavior of firms A transition to another regime may provide the opportunity to use econometric tools such as difference-in-differences to establish the effect of transnational regime (treatment) to the behavior of firms versus the effect of voluntary regime (control/counterfactual). Researchers can also look at the aspect of corruption in analyzing why labor laws compliance systems fail to protect the rights of workers. Rapid evolution of the labor market due to technological change and the consequent increase of non-standards forms of employment (worthwhile field of further research: labor laws compliance) THANK YOU Hypothesis 1 The normative framework on workers’ rights and labor standards demands different levels of commitment from firms addressed at so many levels – global, national and firm – that tightens the web of rules and reduces the possibility of circumventing labor laws. However, this normative framework did not push state enforcement to a regime that goes beyond self and voluntary regulatory strategies. Contributions Different factors induced the transition from one labor governance regime to another. Firms comply more with the traditional regime of labor regulation than the private labor governance regime. Literature Review How do international and domestic level standards, rules and regulations affect labor governance at the level of state enforcement? Anke Hassel The Evolution of a Global Labor Governance Regime 2008 Case Selection 1. The extent the country has mainstreamed ILS to the national level 2. Prevalence of work-related accidents 3. Transition from a mandatory to a voluntary labor governance regime How international and domestic level standards, rules and regulations affect labor governance at the level of state enforcement Summative Assessment of Firms' Compliance with International Labor Standards Normative Framework on Workers' Rights and Labor Standards The Evolution of Labor Governance and the Changing Behavior of Firms Mechanisms of Enforcement and Compliance with International Labor Standards: What Do They Demand from and Offer to Firms? Summative Assessment of Firms' Compliance with International Labor Standards Miguel Luis U. Arias Why do firms comply with international labor standards? How the mechanisms induced compliance with ILS Theory and Hypotheses Case Selection and Limitations How the demands of the mechanisms correspond with the behavior of firms Unit(s) of Analysis: Labor governance regimes (VEF, LSEF and LLCS) Research Design The Evolution of Labor

Master thesis defense

Transcript: Master thesis defense Pricing process of other works of art How do specialists working at auction houses determine the price estimates of paintings? General conclusion Method Qualitative research method Semi-structured interview Interview questions Collection of data Empirical research Velthuis (2002, 2005) Hypotheses Hypothesis one: specialists working at auction houses use pricing scripts to determine the estimates of paintings. Hypothesis two: specialists pay attention to different price determinants to determine the price estimates of paintings. Hypothesis three: specialists pay attention to different price determinants when they need to determine the price estimates of Old Master paintings and Contemporary paintings. Hypothesis four: specialists pay more attention to artist- or artwork or market- or macro level factors. Hypothesis five: the personal taste of specialists has an influence on the price estimates of paintings. Hypothesis six: potential buyers have the biggest influence on the hammer prices fetched at auction. 'a set of routines which function as a cognitive manual for the variety of pricing decisions that a dealer needs to make in different stages of an artist’s career’ (Velthuis, 2002, p.52). Research questions Confirmed: Pricing scripts are helpful tools to determine price estimates, but potential buyers will always have the final word when it comes to the hammer prices fetched at auction. Hypothesis two: specialists pay attention to different price determinants to determine the price estimates of paintings. Hypothesis three: specialists pay attention to different price determinants when they need to determine the price estimates of Old Master paintings and Contemporary paintings. 1. Do specialists working at auction houses use pricing scripts when they determine the price estimates of paintings? 2. Do experts pay attention to different price determinants when they determine the price estimates of paintings? 3. Do specialists pay attention to different price determinants when they determine the price estimates of Old Master paintings and Contemporary paintings? 4. Which factor has the most influence on the price estimates of paintings according to the specialists? 5. Does the taste of experts has an influence on the price estimates of paintings? 6. How is it possible that hammer prices are not always in line with the price estimates stated by art specialists? Hypothesis one: specialists working at auction houses use pricing scripts to determine the estimates of paintings. Hypothesis five: the personal taste of specialists has an influence on the price estimates of paintings. Hypothesis six: potential buyers have the biggest influence on the hammer prices fetched at auction. Pricing scripts Primary art market versus secondary art market Limitations and recommendations for further research Socially and academically relevant Find out if these experts use ‘pricing scripts’ and if differences can be found in the decision-making process between specialists working at different auction houses. Hypothesis four: specialists pay more attention to artist- or artwork or market- or macro level factors. Determine estimates during the interview Limitation of the research Inspiration and motivation Not entirely confirmed: Priceless or worthless? A qualitative research on how price estimates are determined in the secondary art market Aim and relevance Manou Lourens 319142 Conclusion empirical research Provide a better insight in the pricing process that specialists run through to determine the price estimates, before a painting is put up for auction. Rejected: How do specialists working at auction houses determine the price estimates of paintings? Auction houses in the Netherlands Specialists Interviews Auction houses operating in the UK or China Thank you for your attention!

Master Thesis Defense

Transcript: 10 ' 5 putative lipolytic clones 10E2, 12C11, 14G2, 16G7 and 35H9 http://www.iml.rwth-aachen.de/index.php?id=rotes-meer A A p-nitrophenol 348 nm Thank You Introduction Materials and Methods Results and Discussion Conclusion and Future prospects Acknowledgements Inactive A p-nitrophenylbutyrate (C4) Culture supernatant Tris-HCL (pH 8.0) Sequencing and ORF detection Mining for novel biocatalysts from metagenomes Screening Metagenomic Library for Lipolytic Activity Further characterization of EstATII is required to understand its full potential as a biocatalyst (solvents, heavy metals, enantio- and regioselectivity, expand on substrate specificity). Cloning into an expression vector, followed by over-expression and purification. By Yasmine M. Mohamed Under the supervision of Dr. Rania Siam A Tributyrin agar 37°C 3 days Based on BLASTP analysis and domain search: EstATII is a putative novel lipolytic enzyme p-nitrophenol 410 nm End-repair and A tailing Dr. Rania Siam Dr. Mohamed Ghazy Dr. Ahmed Sayed Mr. Amged Ouf Mr. Hazem Sharaf Dr. Ari Ferreira Dr. Felipe Chambergo Dr. Hamza Al Dorry KAUST Al Alfi Foundation Friends from the Biotechnology Program and specially Rehab Abdallah Lamyaa Shaban Sara Al-alawi Ibrahim Farag Tamer Said Ayman Yehya Applications Atlantis II Deep Hydrolysis Leather Manufacture Waste Treatment Detergents Pulp and Paper Industry Ester Synthesis Pharmaceuticals and cosmetics Biopolymers Biodiesel Transformation 10 minutes Effect of NaCl on EstATII activity Traditionally, biocatalysts were obtained from pure microbial isolates. Less than 1% of microorganisms can be accessed through culture-dependant approaches. A huge potential lies within the majority of the prokaryotic community that is unculturable. p-nitrophenylbutyrate Signal Peptide Detection p-nitrophenylbutyrate Manually placed into 96-well plates Temperature pH NaCl Substrate Specificity p-nitrophenyl palmitate (C16) p-nitrophenylpalmitate (C16) Sample collection and DNA isolation 0.78 0.713 0.72 Mean= 0.737 Dedication Effect of pH on EstATII activity My Family Mohamed Abouelsoud Confirmed by PCR and Sequencing http://www.chemnet.com/dict/dict--1492-30-4--jp.html Pyrosequencing and ORF detection The largest brine pool in the Red Sea and has a maximum depth of 2,194 m and has the highest temperature. Stratified into several layers; the brine-seawater interface, upper convective (UCL), middle convective (MCL) and lower convective layers (LCL). A Continuous demand for novel biocatalysts 10 minutes Culture supernatant Tris-HCl (pH= 8.0) Construction of Phylogenetic Tree 1. Exploring the diversity of microbial communities 2. Discovering novel genes, bioactive compounds and biocatalysts. Characterization of EstATII Substrate Specificity of EstATII MATERIALS AND METHODS Conserved three- dimensional structure A A EstATII Enantio- selectivity Sequence Analysis of EstATII Culture supernatant Tris-HCl (ph=8.0) http://www.cbs.dtu.dk/services/SignalP/ Metagenomics p-nitrophenyl butyrate (C4) The LCL is characterized by Temperature of 68.2°C. pH value of 5.3. Salinity of 270 psu Anoxia. High concentrations of heavy metals. Conserved motifs in the HSL Family Characterization of EstATII revealed: Its thermophilic and halophilic properties which reflect the environment from which it was isolated. It is an esterase not a lipase. Characterization of EstATII Phylogenetic Analysis of EstATII 3'- TC TGG CTG AGC CAT TCG ACT -5' (EstR) Functional Screening for Lipolytic Clones Construction and Screening of Metagenomic Fosmid Library Visualization of Phylogenetic Tree A p-nitrophenol 410 nm http://commonfund.nih.gov/hmp/ Molar concentrations tested: 0M, 0.5M, 1M, 2M, 3M and 4M Ligation Active p-nitrophenol 410 nm http://www.genome.jp/tools-bin/clustalw#clustalw.dnd Substrate Specificity http://asgpb.mhpcc.hawaii.edu/sequence/ http://krse.kaust.edu.sa/mission.html Effect of NaCl Concentration on EstATII Activity Multiple Sequence Alignment http://www.megasoftware.net/ Mining the Atlantis II Deep for Lipolytic Enzymes Effect of pH on EstATII Activity http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi A Family IV: HSL EstATII is a novel esterase isolated from the Atlantis II brine pool. It has thermophilic and halophilic properties. Extreme environments hold great potential for the discovery of novel biocatalysts. Catalytic triad p-nitrophenylbutyrate http://pt.wikipedia.org/wiki/Ficheiro:DNA_ORF.gif 10 minutes Shearing (cc) image by anemoneprojectors on Flickr A Identification and Characterization of a novel thermohalophilic esterase from the Red Sea; the Atlantis II brine pool 0.013 0.015 0.008 Mean= 0.012 A http://www.chemnet.com/dict/dict--1492-30-4--jp.html Identification and Subcloning of Fosmid Harboring EstATII Classification of bacterial lipolytic enzymes into eight families by Arpigny and Jaeger, 1999. Regio- selectivity http://itol.embl.de/ 111 96-well plates 10,656 clones Sequence and Phylogentic Analysis A

Master Thesis Defense

Transcript: Identification of Optimized Variant Calling Pipeline for ONT PCR-Enriched Data Breast Cancer Panel Illumina Report Assessment of Hypotheses Validation Samples: VS 3- Enrichment with PCR ONT Recommended Analysis Pipeline Report 1- Internal Validation (B6) 2- External Validation (GIAB) 1 BRCA1 and BRCA2 Assets Step 4 Step 5 Validation Enrichment with PCR 2 4- Library Preparation and Sequencing 5- Analysis and Comparison 3 Oxford Nanopore How Did We Notice? What are We Doing in a Genetic Cancer Panel? Answer: VARIANTS! A gene variant is a permanent change in the DNA sequence that makes up a gene. These variants CAN cause diseases like CANCER. 3 1 2 Variant-Calling Pipeline Optimization Pipeline Optimization Propositions Branch 1 Targeted Sequencing With Nanopore False Negatives Branch 2 Fast High Depth Long Read 2- Base Quality Score Recalibration 3- Variant Calling Tools 1- PCR Duplication Removal Step 3 Challenge DeepVariant Our Work Cliar3 PEPPER-MARGIN DeepVariant Throughput Cheap Data Handling NanoCaller Medaka TOOLS False Positives Scalability Simple Accuracy 4- Population Allele Frequency Filtering What Has ONT Done for these Errors? Introduction 5- Annotation Tools Section 1 Section 2 ONT Recommended Piplines 2 1 3 ANNOVAR VEP SnpEff Story Step 2 Methods Proposed Steps and Tools to be Tested Final Recommended Pipeline Sequencing Platforms Genome Sequencing Platforms Comparison Where is the Source of Error? 1- First Generation: Sanger Sequencing 2- Next Generation: Illumina Sequencing Polymerase Error and Nanopore Systematic Error Reading and storing the genome as strings. Putting and assembling these strings together by aligning to the reference genome. Step 1 Main Sections Branch 2 3- Third Generation: Nanopore Sequencing Sequencing Applications Wang, Y., Zhao, Y., Bollas, A., Wang, Y., & Au, K. F. (2021). Nanopore sequencing technology, bioinformatics and applications. In Nature Biotechnology (Vol. 39, Issue 11, pp. 1348–1365). Nature Research. https://doi.org/10.1038/s41587-021-01108-x PCR Duplication Removal Population Allele Frequency True Positives False Positives Section 4 Section 3 Acknowledgements! Results Recomme- ndations Key point Key point False Positives Codon Change Amino-Acid Change Base Quality Score Recalibration Variant-Callers Comparison Pipeline Comparison: B1 to B5 True Positives Classifier! Codon Change Amino-Acid Change Mohamad Mahdi Fazlkhani - Winter 1402 - Master Thesis Defense 1- Research Story 2- Research Methodology 3- Research Results 4- Research Recommendations

Master Thesis - Defense Presentation

Transcript: Findings H1: 'Playing a game' (X) fosters co-creation (Y) in an artwork-event H2: Participant-centered approach in Gamified Interaction (X) is a necessary condition to the occurrence of a gamified artwork-event (Y) Connection to art Dominique Vinckenbosch "How does Gamified Interaction allow participants to co-create digital artworks?" Thank you Results Media Arts: contexts of games, HCI and interactive art Qualitative analysis: comparative method, within-case method of analysis and embedded multiple-case study August 29, 2019 H1 confirmed H2 rejected Master Thesis Defense Presentation Analysis Gamified Interaction How does Gamified Interaction allow participants to co-create digital artworks? "The deliberate application of game design elements to interaction (or interaction-passing reaction or tricked interaction) taking place between two parties (respectively a human audience and the other one being of digital nature), and from which will result an artwork-event. The implementation process is made according to the audience's personal expectations of a successful interaction (art) experience"

Master Thesis Defense

Transcript: Introduction: What is the puzzle? Public Goods Good Performance of Local Governance Without Democracy? A Case Study of A Farmers’ Specialized Cooperatives and Public Goods Provision in Rural China ---By Yi Liu

Master thesis Defense

Transcript: Party who has to bear the costs of reparations Wrongful State vs. convicted offender Lengthy processes Recognition primarily 'on paper'? Research question How is the emerging international recognition of collective reparations anchored in law: International public law International human rights law International humanitarian law International criminal law Quasi-legal instruments Vague language in most treaties and legislation Interpretation by treaty bodies, courts and commissions Master Victimology and Criminal Justice & Research Master in Law Regional differences Indigenous peoples Human right vs. criminal law Most instruments recognize both, except: Binding sources collective reparations To undo or diminish the suffered harm Awarded to collectives Material and symbolic Different branches of internation law International public law Human rights law and criminal law Quasi-legal instruments Different instruments Binding and non-binding legal sources Court rulings Recommendations Wide variety of reparative measures Recognition of collective reparations Alarming observations Several observations were made: The Recognition of Collective Reparations in International Law and Quasi-legal Instruments 20th century vs. 21th century Except regional human rights law Questions? Manon Bax Several preliminary observations were made More research is needed Master Thesis Often collective reparations in cases involving indigenous peoples Most indigenous peoples have collective background Master Thesis Defense Individualism vs. collectivism Liberalism vs. communitarianism Western vs. non-Western Difference in material needs Cultural and ideological background Discussion Discussion Material vs. symbolic Development over time Margin of appreciation

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